光栅型多功能读板仪 您所在的位置:

光栅型多功能读板仪

设备归属 : 生命平台

设备管理员联系方式 :

秦苏,qins@sustc.edu.cn,8801 5855

收费标准 : 校内 :    校外 :   

阅读资料

设备介绍 :

设备品牌:

PerkinElmer EnSpire

Specifications

General


Wavelength   selection

Quad monochromators

Plate format

6- to 384-well plates 

Temperature control


   Termperature   range

3° C above ambient to 65° C (0.5 °C   increments)

   Uniformity

+0.5° C

Shaking

Linear, orbital, double orbital

Typical throughput (time per plate)

96 well: 31 seconds 
  384 well: 66 seconds

Single read – on-the-fly (time per plate)

96 well: 22 seconds 
  384 well: 27 seconds

Barcode reader

All four sides of the microplate

Dispenser


  Volume range

1 – 475μl per stroke, multiple dispense strokes can   be programmed

 Dispense increments

0.5μl steps

  Speed

50-500μl/sec

    Dead volume (i.e. reagent volume needed to prime the system)

400μl with pump back function


Detection limit specifications with default   settings

Fluorometry   (Fluorescein)


   96-well   plate

< 1 fmol/well

   384-well plate

< 1 fmol/well

   Bottom detection 384-well   plate

< 5 fmol/well

   Monochromator wave length   range

 230-850 nm 0.1 nm step

   Monochromator bandwidth   (Exc & Em)

 < 5 nm

Time Resolved Fluorescence (Xenon flash   lamp)


  96-well plate

<32 amol/well

   384-well   plate

<6  amol/well

Photometry (Filter   or Monochromators)


   96-well plate

0-4 OD

   384-well plate

0-4 OD

   Accuracy   @ 2 optical density

< 2 %

   Precision   @ 2 optical density

< 0.1 %

   Monochromator   wavelength range

230-1000 nm 0.1 nm   step

   Monochromator   bandwidth

<5 nm

Luminometry(Standard)


   96-well   plate ATP

< 10 pM (Glow)

   384-well   plate ATP

< 10 pM (Glow)

   384-well   plate ATP

< 15 amol/well   (Flash)